Transcriptome analysis of six tissues obtained post-mortem from sepsis patients (Journal of Cellular and Molecular Medicine - 2023)
Abstract: Septic shock is a life-threatening clinical condition characterized by a robust immune inflammatory response to disseminated infection. Little is known about its impact on the transcriptome of distinct human tissues. To address this, we performed RNA sequencing of samples from the prefrontal cortex, hippocampus, heart, lung, kidney and colon of seven individuals who succumbed to sepsis and seven uninfected controls. We identified that the lungs and colon were the most affected organs....
Hemopexin and Albumin inhibit heme-induced macrophage activation while also enabling heme-LPS synergistic promotion of TNF production (Advances in Redox Research - 2023)
Abstract: Free heme released from hemoglobin contributes to exacerbated inflammation and tissue damage in hemolytic diseases. While a moderate level of free heme does not cause intravascular inflammation by itself, its presence during infection greatly enhances inflammation. Although specific serum proteins have been found to affect heme-induced inflammation, the selective contribution of serum proteins inhibiting macrophage activation by heme or, conversely, amplifying the production of cytokines by macrophages stimulated with heme and microbial molecules, is poorly defined....
Polycomb Repressive Complex 1 subunit Cbx4 positively regulates effector responses in CD8 T cells (The Journal of Immunology - 2023)
Abstract: CD8 T cell differentiation is controlled by the crosstalk of various transcription factors and epigenetic modulators. Uncovering the different players in regulating this process is fundamental to improving immunotherapy and designing novel therapeutic approaches. Here, we show that Polycomb Repressive Complex (PRC)1 subunit Chromobox (Cbx)4 favors differentiation to effector CD8 T cells. Cbx4 deficiency in CD8 T cells induced transcriptional signature and phenotype of memory cells, increasing the formation of memory population during acute viral infection....
Ten Quick Tips for Harnessing the Power of ChatGPT/GPT-4 in Computational Biology (Under review - PLOS Computational Biology - 2023)
Abstract: The rise of advanced chatbots, such as ChatGPT, has stirred excitement and curiosity in the scientific community. Powered by large language models (LLMs) GPT-3.5 and GPT-4, ChatGPT is a General Purpose Technology with the potential to impact the job market and research endeavors in numerous fields [1]. Although similar models have been fine-tuned for biology-specific projects, including text-based analysis and biological sequence decoding [2,3], ChatGPT provides a natural interface for bioinformaticians to begin using LLMs in their activities....
Gene Signatures of Symptomatic and Asymptomatic Clinical Immunological Profiles of Human Infection by Leishmania L. Chagasi in Amazonian Brazil (Microorganisms - 2023)
Abstract: Individuals infected with Leishmania (L.) chagasi may present different asymptomatic and symptomatic stages of infection, which vary in the clinical鈥搃mmunological profiles that can be classified as asymptomatic infection (AI), subclinical resistant infection (SRI), indeterminate initial infection (III), subclinical oligosymptomatic infection (SOI), and symptomatic infection (SI) (=American visceral leishmaniasis, AVL). However, little is known about the molecular differences between individuals having each profile. Here, we performed whole-blood transcriptomic analyses of 56 infected individuals from Par谩 State (Brazilian Amazon), covering all five profiles....